To remove pet urine smell from a mattress, start by pressing a clean, dry cloth or paper towel onto the affected area to absorb as much urine as possible. Avoid scrubbing, since it can spread the urine and push it deeper into the mattress layers. Urine odors can linger in mattresses because liquid may soak below the surface, leaving behind odor-causing residue even after the fabric feels dry. That’s why a bio-enzyme cleaner can be helpful, since enzymes work to break down the source of the odor instead of simply covering it with fragrance.

For a deeper clean, apply a bio-enzyme stain and odor eliminator according to the product directions, using enough to treat the affected area without oversaturating the mattress. RUFF STUFF Bio-Enzyme Stain & Odor Eliminator by Lillian Ruff is designed to target organic, protein-based, and fat-based pet messes, including urine, feces, vomit, saliva, food spills, and odors. Because mattress materials can vary, always test on a hidden area first, allow the product time to work as directed, blot away excess moisture, and let the mattress dry completely before putting bedding back on.