How long an enzyme cleaner should sit depends on the surface, the type of mess, and how long the stain or odor has been there, so always follow the product directions first. As a general guideline, enzyme cleaners usually need about 10 to 15 minutes of dwell time for light or fresh stains, while older, deeper, or stronger-smelling pet messes may need 30 minutes or longer so the enzymes have more time to work on the organic residue. The goal is to give the cleaner enough contact time to reach the odor source instead of wiping it away too quickly.

For best results, apply the cleaner to the affected area without oversaturating, allow it to sit as directed, then blot or wipe away any excess product if needed. RUFF STUFF Bio-Enzyme Stain & Odor Eliminator by Lillian Ruff is designed to target stains and odors at the source, with enzymes that work on organic residue from pet accidents, urine, feces, vomit, saliva, food spills, and other household odors. Since surfaces can vary, always test on a hidden area first and avoid using enzyme cleaner on materials that are not recommended by the product label.